DNtegrAte: An Open-Source Python Package for Performing DNA Assemblies with the OT-2 Liquid Handling Robot |
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Abstract: DNA assembly is low throughput and unreliable. Implementation of DNA design software is usually done by hand which is low-throughput and error-prone. Here, we present an open-source python package to integrate DNA assembly software with the OT-2 liquid handling robot. Our package reduces the time, training, costs, and wastes associated with molecular biology. We demonstrate the flexibility and versatility of DNtegrAte by demonstrating PCRs and multipart assemblies.